Rat Collagen Type Ⅱ (COL2) ELISA Kit

Cat.#:

JL25981

Regulatory Status:

RUO

Alternative:

COL2

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.62-40ng/mL

Sensitivity:

0.24ng/mL

Specificity:

This kit recognizes Rat COL2 in samples. No significant cross-reactivity or interference between Rat COL2 and analogues was observed.

Sample Type:

Serum;plasma;tissue homogenates and other biological fluids

Assay Procedure:

Research Area:

Metabolism

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| COL2

Type II collagen is the basis for hyaline cartilage, including the articular cartilages at joint surfaces. It is formed by homotrimers of collagen, type II, alpha 1 chains.It makes up 50% of all protein in cartilage and 85–90% of collagen of articular cartilage.Type II collagen is organised into fibrils. This fibrillar network of collagen allows the cartilage to entrap the proteoglycan aggregate, as well as providing tensile strength to the tissue. Oral administration of native type II collagen induces oral tolerance to pathological immune responses and may be useful in arthritis.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Rat COL2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-105	102
Tissue Homogenates(n=8)	95-106	104

| Linearity

Samples were spiked with high concentrations of Rat COL2 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Tissue Homogenates(n=4)
1 : 2	Range (%)	84-95	88-96	85-94
1 : 2	Average (%)	91	93	92
1 : 4	Range (%)	89-103	87-108	85-109
1 : 4	Average (%)	94	98	96